Oriented and sustained protein expression on biomimicking electrospun fibers for evaluating functionality of cells

Abstract A proper protein orientation is often required in order to achieve specific protein-receptor interaction elicit a desired biological response. Here, we present Protein A-based biomimicking platform that capable of efficiently orienting proteins for evaluating cellular behaviour. By absorbing onto aligned bio-mimicking polycaprolactone (PCL) fibers, demonstrate binding could be retained on these fibers at least 7 days under physiologically relevant conditions. We further show served as molecular orientor arrange the recombinant similar orientations. Such protein-orienting scaffolds were verified biologically functional by using sensitive primary rat cortical neurons (CNs) and oligodendrocyte progenitor cells (OPCs), model neural stringent proof concept. Specifically, CNs seeded coated with known enhancer neurite growth (L1 Cell Adhesion Molecular L1CAM) displayed longest total length (462.77 ± 100.79 ?m, p